Caspase-9 Colorimetric Assay Kit (BN00060)

SKU:
BN00060
€295

Description

ELISA Kit Technical ManualMSDS

Caspase-9 Colorimetric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Key Information Description

Detection Method

Absorbance (400 or 405 nm)

Size Options

  • 25 Assays
  • 100 Assays
  • 200 Assays
  • 400 Assays

Species Reactivity

Mammalian

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits

  • Simple one-step procedure; takes 1-2 hours
  • Fast and convenient
  • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.

Kit Components

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • LEHD-pNA (4 mM)
  • DTT (1 M)
  • Dilution Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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