Cathepsin H Activity Fluorometric Assay Kit (BN00425)

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SKU:
BN00425
¥67,982.61

Description

ELISA Kit Technical ManualMSDS

Cathepsin H Activity Fluorometric Assay Kit

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-H Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-H substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-H will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

Figure: Cathepsin H Activity Assay. Cathepsin H assays were performed using various numbers of Jurkat cells (A) or various amounts of purified human liver cathepsin H (B), as indicated. Results were analyzed using a fluorescence plate reader (Ex/Em = 400/505 nm) as described in the kit instructions.

Key Information Description

Product SKU

BN00425

Size

100 assays

Detection Method

Fluorescence (Ex/Em 400/505)

Species Reactivity

Mammalian

Applications

Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.

Features and Benefits

  • Simple one-step procedure; takes only 1-2 hours
  • Fast and convenient
  • Cathepsin-H assay is straightforward, and can be adapted to 96-well plate assays.

Kit Components

  • CH Cell Lysis Buffer
  • CH Reaction Buffer
  • CH Substrate R-AFC (10 mM)
  • CH Inhibitor (1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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