Cystathionine Beta Synthase Inhibitor Screening Kit (Fluorometric) (BN00919)

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SKU:
BN00919
€708

Description

ELISA Kit Technical ManualMSDS

Cystathionine Beta Synthase Inhibitor Screening Kit (Fluorometric)

Cystathionine Beta Synthase (EC 4.2.1.22, CBetaS) is a Pyridoxal 5-Phosphate-dependent enzyme that uses cysteine and homocysteine as substrates to catalyze the formation of H2S and cystathionine. CBetaS is well-known for its role in human sulfur metabolism. The overexpression of CBetaS has been implicated in Down Syndrome, and also results in homocystinuria; or low levels of homocysteine in blood. Homocystinuria leads to a mutli-systemic disorder of the connective tissue, muscles, and central nervous system (CNS). For that reason, pharmacological inhibitors of human CBetaS present attractive therapeutic potential in order to restore baseline CBetaS activity and blood homocysteine levels. It has been shown that aminooxyacetic acid (AOAA) is an irreversible inhibitor that targets the PLP-binding site of CBetaS. That interaction inhibits the transaminase reaction that would yield cystathionine and H2S in wildtype conditions. Assay Genie's Cystathionine Beta Synthase Inhibitor Screening Kit is the first available kit that enables customers to screen inhibitors of CBetaS and quantify their therapeutic potential. The kit has a simple, easy-to-follow protocol and is high-throughput adaptable. In this kit, the product hydrogen sulfide reacts with the non-fluorescent azido-functional group to yield a fluorescent amino group (Ex/Em = 368/460 nm). In the presence of AOAA, CBetaS activity is inhibited, which subsequently abolishes the production of H2S and thus the fluorescent signal is reduced.

Figure: Inhibition of CβS activity by AOAA Inhibitor Control. IC 50 was determined to be 8.0 ± 0.11 µM. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00919

Size

100 assays

Detection Method

Fluorescence (Ex/Em 368/460 nm)

Application

Screening and characterization of inhibitors of Cystathionine Beta Synthase (CBetaS)

Features and Benefits

  • Simple and High throughput adaptable
  • Fluorometric Detection
  • Screen within 60 min

Kit Components

  • CBetaS Assay Buffer
  • CBetaS Probe (in DMSO)
  • CBetaS Substrate
  • Cofactor 1
  • Cofactor 2
  • Reducing Agent
  • CBetaS
  • AOAA (100 mM in DMSO)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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