Cytochrome c Releasing Apoptosis Assay Kit (BN00525)

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SKU:
BN00525
€768

Description

ELISA Kit Technical ManualMSDS

Cytochrome c Releasing Apoptosis Assay Kit

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. Assay Genie's Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.

Key Information Description

Detection Method

BN00525

Size

100 assays

Detection Method

Western blotting

Species Reactivity

Mammalian

Applications

Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.

Features and Benefits

  • Simple procedure; takes ~3 hours
  • Fast and convenient
  • Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.

Kit Components

  • Mitochondria Extraction Buffer
  • 5X Cytosol Extraction Buffer
  • DTT (1 M)
  • 500X Protease Inhibitor Cocktail
  • Anti-Cytochrome c mouse mAb (0.2 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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