|Product Name:||E.Coli GLK E.coli Recombinant Protein|
|Synonyms:||Glucokinase, Glucose kinase, glk, b2388, JW2385.|
|Physical Appearance:||Sterile Filtered colorless solution.|
|Formulation:||GLK protein solution (1.0 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.|
|Stability:||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.|
|Purity:||Greater than 95% as determined by SDS-PAGE.|
|Amino Acid Sequence:||MGSS HHHH HHSS GLVP RGSH MGSM TKYA LVGD VGGT NARL ALCD IASG EISQ AKTY SGLD YPSL EAVI RV Y LEEH KVEV KDGC IAIA CPIT GDWV AMTN HTWA FSIA EMKK NLGF SHLE IIND FTAV SMAI PMLK KEHL I QF GGAE PVEG KPIA VYGA GTGL GVAH LVHV DKRW VSLP GEGG HVDF APNS EEEA IILE ILRA EIGH VSAE RVLS GPGL VNLY RAIV KADN RLP ENLK PKDI TERA LADS CTDC RRAL SLFC VIMG RFGG NLAL NLGT FGGV FIAG GIVP RFLE FFKA SGF RAAF EDKG RFKE YVHD IPVY LIVH DNPG LLGS GAHL RQTL GHIL|
Glucokinase also known as GLK is a member of the bacterial glucokinase family. GLK is not highly significant in E.coli since glucose is already transported into the cell through the PTS system as glucose 6-phosphate.
GLK E.coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 344 amino acids (1-321 a.a) and having a molecular mass of 37.1kDa. GLK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.