Fluorescein Active Caspase-9 Staining Kit (BN00461)

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SKU:
BN00461
€631

Description

ELISA Kit Technical ManualMSDS

Fluorescein Active Caspase-9 Staining Kit

Activation of caspases plays a central role in apoptosis. The Fluorescein Active Caspase-9 Staining Kit provides a convenient means for sensitive detection of activated caspase-9 inLiving cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.

Key Information Description

Detection Method

Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)

Size Options

  • 25 Assays
  • 100 Assays

Species Reactivity

Mammalian

Applications

Sensitive detection of activated caspase-9 in living cells.

Features and Benefits

  • Simple one-step procedure; takes only 1-2 hours
  • Fast and convenient
  • The FITC label allows for direct detection of the activated caspases in apoptotic cells

Kit Components

  • FITC-LEHD-FMK
  • Wash Buffer
  • Z-VAD-FMK

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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