Free Fatty Acid Quantification Colorimetric/Fluorometric Kit (BN00836)

SKU:
BN00836
Product type:
Cellular Assay
  • Cell Biology Assays Free Fatty Acid Quantification Colorimetric/Fluorometric Kit BN00836
  • Cell Biology Assays Free Fatty Acid Quantification Colorimetric/Fluorometric Kit BN00836
  • Cell Biology Assays Free Fatty Acid Quantification Colorimetric/Fluorometric Kit BN00836
  • Cell Biology Assays Free Fatty Acid Quantification Colorimetric/Fluorometric Kit BN00836
€732

Description

ELISA Kit Technical ManualMSDS

Free Fatty Acid Quantification Colorimetric/Fluorometric Kit

Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. Assay Genie's Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples.

Figure: Standard Curves using kit protocol: colorimetric (a), fluorometric (b). Quantitation of fatty acids in human saliva (c) and rat liver lysate (d).

Key Information Description

Product SKU

BN00836

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The kit has a detection limit 2 µM free fatty acid in variety samples.

Features and Benefits

  • Simple procedure; takes ~40 minutes
  • Fast and convenient
  • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Kit Components

  • Fatty Acid Assay Buffer
  • Fatty Acid Probe (in DMSO)
  • ACS Reagent
  • Enzyme Mix
  • Enhancer
  • Palmitic Acid Standard (1nmol/µl)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.