Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric) (BN00686)

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BN00686
  • Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric) (BN00686)
  • Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric) (BN00686)
  • Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric) (BN00686)
€499

Description

ELISA Kit Technical ManualMSDS

Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells. Gelatinase activity is usually detected by small peptide-based activity assays which may suffer from lack of substrate specificity. Other methods for gelatinase activity include gelatin Zymography where samples are electrophoresed on a gelatin-containing SDS-PAGE, and further renatured in a suitable buffer for 12-16 h. The zymogram is subsequently stained, and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. Such methods are laborious, time-consuming and may lead to the loss of enzymatic activity as renaturation may not be completely reversible. Assay Genie's Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU for bacterial collagenase) in biological samples.

Figure: (A) FITC Standard Curve, (B) Gelatinase activity with different amounts of Enzyme Positive Control and (C) in rat heart, kidney lysates along with Hela and U937 cell lysates are shown in the figure (n = 3). The assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN00686

Size

100 assays

Kit Summary

  • Detection method- Fluorometric (490/520 nm)
  • Species reactivity- mammalian
  • Applications-Measurement of gelatinase activity- For screening/studying/characterizing gelatinase inhibitors

Detection Method

Fluorescence (490/520 nm)

Species Reactivity

Mammalian

Applications

  • Measurement of gelatinase activity
  • For screening/studying/characterizing gelatinase inhibitors

Features and Benefits

  • Rapid & sensitive
  • Simple assay to measure gelatinase activity as well as to screen/study/characterize potential inhibitors of gelatinase
  • Includes Fluorometric Gelatinase Substrate and Positive Control

Kit Components

  • Gelatinase Assay Buffer
  • Cell Lysis Buffer
  • Enzyme Positive Control
  • Gelatinase (Substrate)
  • FITC Standard (5 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.v

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Additional Information

Product type:
Assay
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