Glucagon (human/mouse/rat) EIA Kit (BN00710)

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ELISA Kit Technical ManualMSDS

Glucagon (human/mouse/rat) EIA Kit

Assay Genie's Glucagon Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Glucagon antibody, both biotinylated Glucagon peptide and peptide standard or targeted peptide in samples interacts competitively with the Glucagon antibody. Uncompeted (bound) biotinylated Glucagon peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Glucagon peptide in the standard or samples. This is due to the competitive binding to Glucagon antibody between biotinylated Glucagon peptide and peptides in standard or samples. A standard curve of known concentration of Glucagon peptide can be established and the concentration of Glucagon peptide in the samples can be calculated accordingly. The minimum detectable concentration of Glucagon is 0.97 pg/ml. The detection range for the kit is 1-1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

Figure: Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Key Information Description

Product SKU



100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Human, mouse, rat


Quantitative protein detection, establishing normal range, validation of antibody array results.

Features and Benefits

  • Easy, convenient and time-saving method to assay for Glucagon.
  • The minimum detectable concentration of Glucagon is typically less than 0.97 pg/ml.
  • This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Kit Components

  • Glucagon Microplate
  • Wash Buffer Concentrate (20x)
  • Lyophilized standard Glucagon peptide
  • Lyophilized anti-Glucagon polyclonal antibody
  • 5X Assay Diluent B
  • Lyophilized biotinylated Glucagon peptide
  • HRP-Streptavidin concentrate
  • Lyophilized positive control
  • TMB One-Step Substrate Reagent
  • Stop Solution

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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