Glucose-6-Phosphate Colorimetric Assay Kit (BN00881)

SKU:
BN00881
€589

Description

ELISA Kit Technical ManualMSDS

Glucose-6-Phosphate Colorimetric Assay Kit

Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. Assay Genie's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450 nm. The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P.

Figure: Glucose-6-phoshate standard curve generated using this kit protocol

Key Information Description

Product SKU

BN00881

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Application

  • The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P.

Features and Benefits

  • Simple procedure; takes ~ 40 minutes
  • Fast and convenient
  • Kit contains all necessary reagents for accurate measurement of Glucose-6-Phosphate (G6P)

Kit Components

  • G6P Assay Buffer
  • G6P Enzyme Mix
  • G6P Substrate Mix
  • G6PStandard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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