Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00964)

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BN00964
  • Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00964)
  • Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00964)
  • Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (BN00964)
€708

Description

ELISA Kit Technical ManualMSDS

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)

Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. Assay Genie's Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 µU of G6PDH activity.

Figure: (a) NADPH Standard Curve. (b) Kinetic measurement of G6PDH activity in various samples. (c) G6PDH specific activity was calculated in lysates prepared from MCF-7 (0.29 µg), MDA-MB-468 (0.41 µg), rat liver (0.6 µg) and orchid leaf (0.56 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00964

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

  • Measurement of G6PDH activity in various tissues and cells
  • Evaluation of pentose phosphate pathway

Features and Benefits

  • Rapid & simple
  • High-throughput adaptable
  • Can detect as low as 1 µU of G6PDH activity

Kit Components

  • G6PDH Assay Buffer (in DMSO)
  • G6PDH Substrate
  • G6PDH Developer
  • G6PDH Positive Control
  • NADPH Standard (200 nmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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