Glutamate Dehydrogenase Activity Colorimetric Assay Kit (BN00948)

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SKU:
BN00948
  • Glutamate Dehydrogenase Activity Colorimetric Assay Kit (BN00948)
  • Glutamate Dehydrogenase Activity Colorimetric Assay Kit (BN00948)
€553

Description

ELISA Kit Technical ManualMSDS

Glutamate Dehydrogenase Activity Colorimetric Assay Kit

Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes. Assay Genie's GDH Assay Kit provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (= 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

Figure: NADH Standard Curve obtained using kit protocol and GDH timeline graph. *Sample 01: Bovine Liver extraction (2 µg protein), Sample 02: 5 µl Rabbit serum

Key Information Description

Product SKU

BN00948

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assays for measuring GDH activity in various biological samples and the kit is also suitable for high throughput analyses.

Kit Components

  • GDH Assay Buffer
  • Glutamate (2 M)
  • GDH Developer
  • GDH Positive Control
  • NADH (0.5 ol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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