Glutamine Colorimetric Assay Kit (BN00786)

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SKU:
BN00786
  • Glutamine Colorimetric Assay Kit (BN00786)
  • Glutamine Colorimetric Assay Kit (BN00786)
  • Glutamine Colorimetric Assay Kit (BN00786)
€673

Description

ELISA Kit Technical ManualMSDS

Glutamine Colorimetric Assay Kit

Glutamine (Gln) is one of the most abundant amino acids containing an uncharged amide as a side chain. It is synthesized via condensation of glutamate and ammonia. Gln is classified as a non-essential amino acid, however, Gln is important in several biological processes such as protein synthesis, regulation of acid-balance in mammalian kidneys and cell growth. It constitutes cell's main source of nitrogen for the synthesis of nucleotides and hexosamines. Glutamine-rich diets benefit patients suffering from Crohn's disease, severe burns, HIV/AIDS and cancer. Assay Genie's Glutamine Colorimetric Assay kit is a simple and sensitive assay that detects the biologically relevant concentrations of Gln in various fluids and tissues. The assay is based on the hydrolysis of Glutamine to Glutamate producing a stable signal, which is directly proportional to the amount of Gln in the sample. The assay can detect as little as 25 µM of Gln in a variety of biological samples.

Figure: (a) Glutamine Standard Curve, (b) Measurement of Glutamine concentration in human urine (5 µl), (c) Measurement of Glutamine concentration in mouse tissues (brain [15µg], liver [50 µg]). Samples were deproteinized using 10K Spin Column spiked with known amount of Glutamine (6 nmol). Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00786

Size

100 assays

Detection Method

Absorbance (450 nm)

Applications

Measurement of Glutamine in various biological samples

Features and Benefits

  • Convenient & Rapid Protocol
  • This kit can detect as little as 25 µM of Glutamine in a variety of biological samples

Kit Components

  • Hydrolysis Buffer
  • Development Buffer
  • Hydrolysis Enzyme Mix (Lyophilized)
  • Development Enzyme Mix (Lyophilized)
  • Developer (Lyophilized)
  • Gln Standard (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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