Granzyme B Activity Fluorometric Assay Kit (BN00448)



ELISA Kit Technical ManualMSDS

Granzyme B Activity Fluorometric Assay Kit

Granzyme B (GZMB, EC number also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In Assay Genie's Granzyme B Activity Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

Figure: AFC Standard

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em = 380/500 nm)


Measurement of Granzyme B activity in various biological samples or purified Granzyme B activity, Screening Granzyme B inhibitors, characterize/Study Granzyme B inhibitors.

Features and Benefits

  • Simple and Sensitive
  • Rapid and convenient
  • Kit contains all necessary reagents for measuring Granzyme B Activity.

Kit Components

  • Granzyme B Assay Buffer
  • Granzyme B Substrate
  • Positive Control (Granzyme B Enzyme, human recombinant)
  • AFC Standard (1mM)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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