null

[KD Validated] Smad4 Polyclonal Antibody

SKU:
CAB21487
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $635
Frequently bought together:

Description

Product Name:[KD Validated] Smad4 Polyclonal Antibody
Product Code:CAB21487
Reactivity:Human, Mouse
Applications:Western blotting
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Reactivity:Human, Mouse
Tested Applications:WB, ELISA
Key Applications:Western blotting
Recommended Dilution:WB 1:500-1:1000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Positive Samples:293T, HCT116
Cellular Location:Cytoplasm, Nucleus

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-450 of human Smad4 (NP_005350.1).
Sequence:DEYVH DFEGQ PSLST EGHSI QTIQH PPSNR ASTET YSTPA LLAPS ESNAT STANF PNIPV ASTSQ PASIL GGSHS EGLLQ IASGP QPGQQ QNGFT GQPAT YHHNS TTTWT GSRTA PYTPN LPHHQ NGHLQ HHPPM PPHPG HYWPV HNELA FQPPI SNHPA PEYWC SIAYF EMDVQ VGETF KVPSS CPIVT VDGYV DPSGG DRFCL GQLSN VHRTE AIERA RLHIG KGVQL ECKGE GDVWV RCLSD HAVFV QSYYL DREAG RAPGD AVHKI YPSAY IKVFD LRQCH RQMQQ Q
Synonyms:JIP, DPC4, MADH4, MYHRS
Calculated MW:60kDa
Observed MW:70kDa

Related Products