Lactate Colorimetric Assay Kit (384 Well) (BN01112)

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SKU:
BN01112
  • Lactate Colorimetric Assay Kit (384 Well) (BN01112)
  • Lactate Colorimetric Assay Kit (384 Well) (BN01112)
€637

Description

ELISA Kit Technical ManualMSDS

Lactate Colorimetric Assay Kit (384 Well)

Abnormal high concentration of lactate in blood has been related to many important diseases such as diabetes and lactate acidosis. Elevated Lactate concentration in blood is also associated with shock, post cardiac arrest, tissue ischemia, thiamine deficiency, liver disorder, mitochondrial diseases and many more. Therefore detection and quantitation of lactate in from blood is a diagnostic key for several diseases. Assay Genie's Lactate Colorimetric Assay Kit uses an enzyme mix which reacts with L-Lactate specifically. D-Lactate does not react with the enzyme mix. The enzymatic reaction with L-lactate produces a product that generates a color (OD= 590 nm) when reacts with the Lactate probe. The formed product is directly proportional to the amount of L-Lactate present in the sample. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format.The kit provides a convenient means for detecting 0.5 to 5 mM Lactate in biological samples such as in blood, blood circulation, in cells, in culture media, in fermentation media etc. in 384 HTP format

Figure: (a) Glucose Standard Curve; (b) Quantitation of Glucose in human serum. Serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Undiluted serum filtrate (1 μl) samples were added to the wells directly. Assays were performed according to the kit protocol. Calculated concentration: 86.78 ± 0.1 mg/dl.

Key Information Description

Product SKU

BN01112

Size

384 assays

Detection Method

Absorbance (590 nm)

Species Reactivity

Mammalian

Applications

The kit detects 0.5-500 µM lactate samples

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Lactate Assay Buffer
  • Lactate Probe (in DMSO, anhydrous)
  • Lactate Enzyme Mix (lyophilized)
  • Lactate Standard (100 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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