Lactate Dehydrogenase (LDH: EC 1.1.1.27) is a common enzyme found in wide range of organisms. It catalyzes the interconversion of pyruvate and lactate, with concomitant interconversion of NADH and NAD+. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries. Assay Genie's Lactate Dehydrogenase Activity Assay kit provides a quick and easy method for monitoring Lactate Dehydrogenase activity in a wide variety of samples. In this assay, Lactate Dehydrogenase converts lactate into pyruvate and NADH, which reacts with to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, highly sensitive and high-throughput adaptable, and can detect Lactate Dehydrogenase activity as low as 1 µU/ml.

Figure: (a) NADH Standard Curve, (b) Kinetic measurement of Lactate Dehydrogenase activity in various samples and (c) Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and human serum (0.2 µg protein). Assays were performed following the kit protocol.