Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in thecytoplasm. Assay Genie's Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation

Lysosome Isolation: (a.) Tentative Lysosome band location in gradient density. (b.) Acid Phosphatase (biomarker enzyme of lysosomes) specific activity increased with each purification step. Activity Assay experiments were carried out using Acid Phosphatase Activity Colorimetric Assay Kit. Data is normalized by the amount of protein used per well