Oxalate Oxidase Activity Assay Kit (Fluorometric) (BN00740)

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SKU:
BN00740
  • Oxalate Oxidase Activity Assay Kit (Fluorometric) (BN00740)
  • Oxalate Oxidase Activity Assay Kit (Fluorometric) (BN00740)
  • Oxalate Oxidase Activity Assay Kit (Fluorometric) (BN00740)
€553

Description

ELISA Kit Technical ManualMSDS

Oxalate Oxidase Activity Assay Kit (Fluorometric)

Oxalate Oxidase (OxOx EC 1.2.3.4) belongs to the cupin protein superfamily and catalyzes the conversion of Oxalate into H2O2 and CO2. It plays an important role for stress response in plants. For example, wood rotting fungi generates high levels of oxalate, causing plant rot in many crops including lettuce, soybean, dry bean, tomato, etc. Overexpression of OxOx in plants (e.g. tomato, soybean, lettuce and tobacco) is able to generate transgenic plants showing resistance to fungal pathogenesis. Accurate measurement of Oxalate Oxidase activity is valuable for mechanistic studies in plants and development of transgenic plants. Assay Genie's Oxalate Oxidase Activity Assay kit provides a quick and easy method for the measurement of Oxalate Oxidase activity in various samples. In this assay, Oxalate Oxidase converts oxalate into hydrogen peroxide, which in turn, reacts with a probe and converter generating a fluorometric signal (535/587 nm). The generated fluorescence is directly proportional to the amount of active Oxalate Oxidase present in samples. The assay is simple, sensitive, high-throughput adaptable and can detect less than 4 µU of oxalate Oxidase activity per sample.

Figures. A: H2O2 Standard Curve using this assay protocol. B and C: Oxalate Oxidase (OxOx) activity were measured in Barley Seed Lysate (29 µg) and Barley Sprout Lysate (60 µg). Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00740

Size

100 assays

Kit Summary

  • Detection method: Fluorescence (Ex/Em = 535/587 nm)
  • Application: Measurement of Total Oxalate Oxidase activity in various biological samples.

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Species Reactivity

Plant

Applications

Measurement of Total Oxalate Oxidase activity in various biological samples.

Features and Benefits

  • Rapid & Convenient
  • The assay can detect as little as 4 µU of OxOx enzymatic activity.
  • Includes Active OxOx Enzyme as Positive Control

Kit Components

  • OxOX Assay Buffer
  • OxOx Substrate
  • OxOx Converter
  • GenieRed Probe (in DMSO)
  • OxOX Positive Control
  • H2O2 Standard (0.88 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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