PLTP Activity Fluorometric Assay Kit II (BN00820)

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SKU:
BN00820
  • PLTP Activity Fluorometric Assay Kit II (BN00820)
  • PLTP Activity Fluorometric Assay Kit II (BN00820)
€673

Description

ELISA Kit Technical ManualMSDS

PLTP Activity Fluorometric Assay Kit II

Phospholipid transfer protein (PLTP) plays an important role in transferring phospholipids between HDL molecules to modulate HDL size and composition and controlling plasma HDL levels. PLTP also transfers phospholipids from lipoproteins to HDL. PLTP plays a key role in reverse cholesterol transport and may promote atherosclerosis. PLTP is, therefore, considered a promising target for pharmacological intervention. Assay Genie's PLTP Activity Fluorometric Assay Kit II uses a self-quenched fluorescent phospholipid that can be measured when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of phospholipid transferred. Rabbit serum is provided as a positive control. This Assay Kit, in addition to measuring activity in serum, is also suitable for testing activity of recombinant protein.

                          (a)

                        (b)

Figure: (a) Donor Molecule Standard Curve (b) Measurement of PLTP activity in rabbit serum (1 µl) or human plasma (1 µl).

Key Information Description

Product SKU

BN00820

Size

100 assays

Detection Method

Fluorescence (Ex/Em 465/535 nm)

Applications

Measurement of PLTP activity in animal serum, plasma and recombinant protein

Features and Benefits

  • Rapid and convenient
  • Includes Positive Control (rabbit serum)

Kit Components

  • PLTP Assay Buffer
  • Donor Molecule (30 nmol/ml)
  • Acceptor Molecule
  • Positive Control (rabbit serum)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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