PNPLA8 Antibody (PACO57472)
- SKU:
- PACO57472
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | PNPLA8 Antibody (PACO57472) |
| Antibody SKU: | PACO57472 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Calcium-independent phospholipase A2-γ protein (158-345AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57472 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO57472 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57472 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids. |
| Synonyms: | Calcium-independent phospholipase A2-γ (EC 3.1.1.5) (Intracellular membrane-associated calcium-independent phospholipase A2 gamma) (iPLA2-gamma) (PNPLA-gamma) (Patatin-like phospholipase domain-containing protein 8) (iPLA2-2), PNPLA8, IPLA22 IPLA2G |
| UniProt Protein Function: | PNPLA8: Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; EC 3.1.1.5; Phospholipase; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 7q31Cellular Component: endoplasmic reticulum membrane; intracellular; membrane; peroxisomal membrane; peroxisomeMolecular Function: calcium-independent phospholipase A2 activity; phospholipase A2 activityBiological Process: arachidonic acid metabolic process; arachidonic acid secretion; cell death; fatty acid metabolic process; linoleic acid metabolic process; phosphatidylethanolamine catabolic process; prostaglandin biosynthetic processDisease: Mitochondrial Myopathy With Lactic Acidosis |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the patatin-like phospholipase domain containing protein family. Members of this family are phospholipases which catalyze the cleavage of fatty acids from membrane phospholipids. The product of this gene is a calcium-independent phospholipase. Mutations in this gene have been associated with mitochondrial myopathy with lactic acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2015] |
| UniProt Code: | Q9NP80 |
| NCBI GenInfo Identifier: | 74734299 |
| NCBI Gene ID: | 50640 |
| NCBI Accession: | Q9NP80.1 |
| UniProt Secondary Accession: | Q9NP80,O95035, Q8N3I3, Q9H7T5, Q9NR17, Q9NUN2, Q9NZ79 A4D0S1, C9JZI4 |
| UniProt Related Accession: | Q9NP80 |
| Molecular Weight: | 77,031 Da |
| NCBI Full Name: | Calcium-independent phospholipase A2-gamma |
| NCBI Synonym Full Names: | patatin like phospholipase domain containing 8 |
| NCBI Official Symbol: | PNPLA8Â Â |
| NCBI Official Synonym Symbols: | MMLA; IPLA2G; IPLA2-2; iPLA2gamma; PNPLA-gamma  |
| NCBI Protein Information: | calcium-independent phospholipase A2-gamma |
| UniProt Protein Name: | Calcium-independent phospholipase A2-gamma |
| UniProt Synonym Protein Names: | Intracellular membrane-associated calcium-independent phospholipase A2 gamma; iPLA2-gamma; PNPLA-gamma; Patatin-like phospholipase domain-containing protein 8; iPLA2-2 |
| Protein Family: | Calcium-independent phospholipase |
| UniProt Gene Name: | PNPLA8Â Â |
| UniProt Entry Name: | PLPL8_HUMAN |
| Antibodies |
| PNPLA8 Antibody (PACO00978) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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