Proteasome Activity Fluorometric Assay Kit (BN00510)

(No reviews yet) Write a Review
SKU:
BN00510
  • Proteasome Activity Fluorometric Assay Kit (BN00510)
  • Proteasome Activity Fluorometric Assay Kit (BN00510)
€553

Description

ELISA Kit Technical ManualMSDS

Proteasome Activity Fluorometric Assay Kit

Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes) which are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. Assay Genie's Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes, which make the assay specifically measures proteasome activity from other protease activity which may be present in samples.

Figure 1: AMC Standard Curve and Proteasome Activity assay using the kit protocol: A: AMC standard curve 0-100 pmole; B: Kinetics of Proteasome Activity assay in the absence (1) and presence (2) of MG-132 Proteasome inhibitor. Equations represent best fit of lines during the linear portion of the reaction (after ~ 25 min in this case).

Key Information Description

Product SKU

BN00510

Size

100 assays

Detection Method

Fluorescence (Ex/Em 350/440nm)

Species Reactivity

Mammalian

Applications

Specifically measures proteasome activity from other protease activity present in samples.

Features and Benefits

  • Simple one-step procedure; takes only 30-60 minutes
  • Accurate and easy to use

Kit Components

  • Proteasome Assay Buffer
  • Proteasome Substrate (In DMSO)
  • Proteasome Inhibitor (In DMSO)
  • AMC Standard (1 mM in DMSO)
  • Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose

Additional Information

Product type:
Assay
View AllClose