QuickStep Human BMG/β2-MG (Beta-2-Microglobulin) ELISA Kit

Product Name:	QuickStep Human BMG/β2-MG (Beta-2-Microglobulin) ELISA Kit
Product Code:	AEES00633
Assay Type:	Sandwich
Format:	96 Assays
Assay Time:	2.5h
Reactivity:	Human
Detection Range:	0.78-50 ng/mL
Sensitivity:	0.47 ng/mL
Sample Type & Sample Volume:	Serum, plasma, urine, 50μL
Specificity:	This kit recognizes Human BMG/β2-MG in samples. No significant or interference between Human BMG/β2-MG and analogues was observed.
Reproducibility:	Both intra-CV and inter-CV are < 10%.
Application:	This ELISA kit applies to the in vitro quantitative determination of Human BMG/β2-MG concentrations in serum, plasma, urine.Please consult technical support for the applicability if other biological fluids need to be tested.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMG/β2-MG. Samples (or Standards) and biotinylated detection antibody specific for Human BMG/β2-MG are added to the micro ELISA plate wells. Human BMG/β2-MG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMG/β2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human BMG/β2-MG. You can calculate the concentration of Human BMG/β2-MG in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Item	Specifications	Storage conditions after test
Micro ELISA Plate (Dismountable)	8 wells x 12 strips	2-8℃, 1 month
Reference Standard	2 vials	Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃
Biotinylated Detection Ab Working Solution	1 vial, 6 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	2-8℃ (Protect from light)
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

1.	Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2.	Aspirate and wash the plate for 3 times
3.	Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4.	Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5.	Add 50μL Stop Solution
6.	Read the plate at 450nm immediately. Calculation of the results

Beta 2 microglobulin (β2M) is a small protein (11,800 Dalton), normally found on the surface of many cells, including lymphocytes, and in small amounts in the blood and urine [1]. An increased amount in the blood or urine may be a sign of certain diseases, including some types of cancer, such as multiple myeloma or lymphoma.β2M is critical for the immune surveillance and modulation in vertebrate animals. The dysregulation of β2M is associated with multiple diseases, including endogenous and infectious diseases. β2M could directly participate in the development of cancer cells, and the level of β2M is deemed as a prognostic marker for several malignancies. It also involves in forming major histocompatibility complex (MHC class I or MHC I) or like heterodimers, covering from antigen presentation to immune homeostasis [2].