QuickStep Human ALB (Albumin) ELISA Kit
- SKU:
- AEES00648
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Human
- Sensitivity:
- 0.30 ng/mL
- Range:
- 1.56-100 ng/mL
- Sample Type:
- Serum and plasma
Description
| Product Name: | QuickStep Human ALB (Albumin) ELISA Kit |
| Product Code: | AEES00648 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 2.5h |
| Reactivity: | Human |
| Detection Range: | 1.56-100 ng/mL |
| Sensitivity: | 0.30 ng/mL |
| Sample Type & Sample Volume: | Serum and plasma, 50μL |
| Specificity: | This kit recognizes Human ALB in samples. No significant or interference between Human ALB and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human ALB concentrations in serum and plasma.Please consult technical support for the applicability if other biological fluids need to be tested. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALB. Samples (or Standards) and biotinylated detection antibody specific for Human ALB are added to the micro ELISA plate wells. Human ALB would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human ALB. You can calculate the concentration of Human ALB in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
|
| 1. | Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C |
| 2. | Aspirate and wash the plate for 3 times |
| 3. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 4. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 5. | Add 50μL Stop Solution |
| 6. | Read the plate at 450nm immediately. Calculation of the results |
ALB is the most abundant protein in human plasma and serum. This protein is normally present in serum at concentrations ranging from 30 to 50 mg/mL and accounts for approximately 60% of the total protein content in serum [1]. ALB has a molecular weight of 66.7 kDa and is composed of a single polypeptide chain with 585 amino acids and 17 disulfide bonds. ALB is involved in many physiological processes. For instance, this protein aids in the regulation of osmotic pressure and pH in blood. In addition, ALB mediates lipid metabolism, sequesters toxins, and works as an antioxidant (i.e., it binds free radicals) [2]. Another important function of ALB is its action as a transport protein for a wide range of solutes that include some low mass hormones, fatty acids, and drugs
Related Products
