Rat CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) ELISA Kit (RTES01087)
- SKU:
- RTES01087
Description
Rat CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) ELISA Kit
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CYP1A2 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYP1A2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYP1A2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CYP1A2. The concentration of Rat CYP1A2 in samples can be calculated by comparing the OD of the samples to the standard curve.
| Assay type | Sandwich |
| Format | 96T |
| Assay time | 4.5h |
| Reactivity | Rat |
| Detection Method | Colormetric |
| Detection Range | 0.16-10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Sample Volume Required Per Well | 100uL |
| Sample Type | Serum, plasma and other biological fluids |
Specificity
This kit recognizes Rat CYP1A2 in samples. No significant cross-reactivity or interference between Rat CYP1A2 and analogues was observed.
Typical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| Concentration (ng/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 10 | 2.253 2.267 | 2.26 | 2.169 |
| 5 | 1.476 1.484 | 1.48 | 1.389 |
| 2.5 | 0.863 0.843 | 0.853 | 0.762 |
| 1.25 | 0.438 0.474 | 0.456 | 0.365 |
| 0.63 | 0.276 0.264 | 0.27 | 0.179 |
| 0.32 | 0.199 0.169 | 0.184 | 0.093 |
| 0.16 | 0.135 0.143 | 0.139 | 0.048 |
| 0 | 0.085 0.097 | 0.091 | -- |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CYP1A2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CYP1A2 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.54 | 1.02 | 4.21 | 0.52 | 1.02 | 4.17 |
| Standard deviation | 0.03 | 0.05 | 0.17 | 0.03 | 0.05 | 0.16 |
| C V (%) | 5.56 | 4.90 | 4.04 | 5.77 | 4.90 | 3.84 |
Recovery
The recovery of Rat CYP1A2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 89-100 | 94 |
| EDTA plasma (n=5) | 91-107 | 99 |
| Cell culture media (n=5) | 94-109 | 102 |
Linearity
Samples were spiked with high concentrations of Rat CYP1A2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 96-110 | 92-106 | 85-99 |
| Average (%) | 101 | 100 | 92 | |
| 1:4 | Range (%) | 91-104 | 81-94 | 84-95 |
| Average (%) | 97 | 86 | 90 | |
| 1:8 | Range (%) | 85-98 | 87-100 | 84-97 |
| Average (%) | 92 | 93 | 90 | |
| 1:16 | Range (%) | 90-102 | 85-98 | 83-93 |
| Average (%) | 95 | 90 | 88 |
Kit Components & Storage
An unopened kit can be stored at 4'C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 8 wells X12 strips | -20'C, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100X) | 1 vial, 120 uL | |
| Concentrated HRP Conjugate (100X) | 1 vial, 120 uL | -20'C(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4'C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25X) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 4'C(shading light) |
| Stop Solution | 1 vial, 10 mL | 4'C |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Rat CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) ELISA Kit (RTES01087) Assay procedure
- 1. Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
- 2. Aliquot 100µl of standard solutions into the standard wells.
- 3. Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
- 4. Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids) into test sample wells.
- 5. Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
- 6. Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix.Incubate for 1 hour at 37°C.
- 7. Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
- 8. Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
- 9. Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
- 10. Add 90µL of Substrate Reagent to each well. Cover with a new plate seal and incubate forapproximately 15 min at 37°C. Protect the plate from light. Note: the reaction time can beshortened or extended according to the actual color change, but not by more than 30min.
- 11. Add 50 µL of Stop Solution to each well. Note: Adding the stop solution should be done inthe same order as the substrate solution.
- 12. Determine the optical density (OD value) of each well immediately with a microplate readerset at 450 nm.
Rat CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) ELISA Kit (RTES01087) Protein Information
| UniProt Protein Function: | CYP1A2: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation (By similarity) |
| UniProt Protein Details: | Protein type:Lipid Metabolism - linoleic acid; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Secondary Metabolites Metabolism - caffeine Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle Molecular Function:enzyme binding; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxidoreductase activity; monooxygenase activity; demethylase activity Biological Process: response to drug; dibenzo-p-dioxin metabolic process; cellular respiration; monoterpenoid metabolic process; response to lipopolysaccharide; toxin biosynthetic process; response to organic cyclic substance; monocarboxylic acid metabolic process; response to estradiol stimulus; post-embryonic development; response to organic substance; drug catabolic process; regulation of gene expression; xenobiotic metabolic process; toxin metabolic process; exogenous drug catabolic process; porphyrin metabolic process; aromatic compound metabolic process; alkaloid metabolic process; heterocycle metabolic process; drug metabolic process; hydrogen peroxide biosynthetic process; lung development; steroid catabolic process |
| NCBI Summary: | a monooxygenase that may play a role in xenobiotic metabolism [RGD, Feb 2006] |
| UniProt Code: | P04799 |
| NCBI GenInfo Identifier: | 158186713 |
| NCBI Gene ID: | 24297 |
| NCBI Accession: | NP_036673.3 |
| UniProt Secondary Accession: | P04799,Q64588, A1L120, |
| UniProt Related Accession: | P04799 |
| Molecular Weight: | 58,259 Da |
| NCBI Full Name: | cytochrome P450 1A2 |
| NCBI Synonym Full Names: | cytochrome P450, family 1, subfamily a, polypeptide 2 |
| NCBI Official Symbol: | Cyp1a2 |
| NCBI Official Synonym Symbols: | CYPD45; P-450d; RATCYPD45 |
| NCBI Protein Information: | cytochrome P450 1A2 |
| UniProt Protein Name: | Cytochrome P450 1A2 |
| UniProt Synonym Protein Names: | CYPIA2; Cytochrome P-448; Cytochrome P-450d; Cytochrome P450-D |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | Cyp1a2 |
| UniProt Entry Name: | CP1A2_RAT |
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