PDGFRA; also known as CD140a; together with the structurally homolog protein PDGFRB (CD140b); are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell; and binds all three ligand isoforms PDGF-AA; PDGF-BB and PDGF-AB with high affinity; whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation; chemotaxis and migration of mesangial cells. PDGFRA has been shown to play a role in testis organ development and Leydig cell differentiation, as well as being important for kidney formation. Furthermore, PDGFRA is required for tumor angiogenesis and maintenance of the tumor microenvironment, as well as HCC development and metastasis. PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA amplification rather than gene mutation could be the underlying genetic mechanism driving PDGFRA overexpression in a subset of gliomas. Human PDGFRa/CD140a Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human PDGFRa/CD140a Recombinant Protein (RPES2035)
Product Code:
RPES2035
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD140A,PDGFR-2,PDGFR2,RHEPDGFRA,Platelet-derived growth factor receptor alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor,CD140 antigen-like family member A, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2
Accession:
NP_006197.1
Sequence:
Met 1-Glu 524
Fusion tag:
Activity:
Measured by its ability to bind mouse PDGF-C in functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human PDGFRa (NP_006197.1) extracellular domain (Met 1-Glu 524) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
56.8 kDa
AP Mol Mass:
90-100 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
