10275 results for 'time green'

Time-Resolved Fluorescence (TRF) technology is a cutting-edge analytical technique that has revolutionized the fields of biochemistry, immunology, and molecular biology. By offering a refined appr

Immunodetection · ProtocolsSecondary Antibody Incubation: Times, Conditions and TroubleshootingThe secondary antibody step determines how much signal you get and how much background comes with it. Inc

TUNEL Assay Kits | 1-Step In Situ Apoptosis Detection | Assay Genie

Understanding the dynamics of sodium and potassium ions within biological systems is crucial for deciphering cellular processes and developing therapeutic strategies. Sodium and potassium indicato

Annexin V Apoptosis Assay Kits | Flow Cytometry Detection | Assay Genie

Fluorescent proteins (FPs) have revolutionized the field of molecular and cellular biology, allowing scientists to visualize and track biological processes in live cells with unprecedented clarity

Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual c

Techniques · Apoptosis DetectionTUNEL Staining: The Method of Choice for Measuring Cell DeathTUNEL staining is a gold-standard technique for detecting apoptosis in situ. By labelling the fragmented DN

Methods · FluorescenceFRET Assays: Principle, Förster Radius and Measurement MethodsFRET transfers energy from an excited donor fluorophore to a nearby acceptor without emitting a photon, and the effi

The eukaryotic cell is a complex and dynamic system with distinct membrane-bound compartments known as organelles, each performing specialized functions essential for cellular survival. To better

Apoptosis, or programmed cell death, is a fundamental process that plays a critical role in the development and maintenance of healthy tissues. Central to this process are caspases, a family of cy

The selection of a secondary antibody is a critical step in the experimental design of various immunodetection methods, including Western Blot, ELISA, immunohistochemistry (IHC), and immunofluores

The fight against B cell cancers, a challenging spectrum of hematologic malignancies, has entered a new era with the introduction of rituximab biosimilars. These biosimilars promise to extend the

Metabolism · Biochemistry GuideBeta-Oxidation of Fatty AcidsBeta-oxidation is the mitochondrial pathway that breaks fatty acids down into acetyl-CoA for energy. This guide covers where it happen

Molecular Biology · Techniques Guide Types of PCR – 7 Methods, Principles & Applications PCR stands as a cornerstone in molecular biology, ena

Sample Preparation · ImmunoassaySerum vs Plasma: Key Differences and When to Use EachSerum and plasma come from the same blood draw but are not interchangeable. One is collected after clotting, the ot

In Vivo Methods · ProtocolsMouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous RoutesRoute choice and injection technique determine how much of a dose actually reaches it

At Assay Genie we have created a comprehensive guide outlining everything you need to know about apoptosis! Key Takeaways: Apoptosis is a programmed cell death process, vital for tissue

Chronic myelogenous leukaemia (CML) is a myeloproliferative haematopoietic malignancy, characterised by a karyotypic abnormality present in more than 95% of cases known as the Philadelph

Cell Biology · Programmed Cell DeathApoptosis: Your Complete Guide to Intrinsic & Extrinsic PathwaysApoptosis is programmed cell death — the controlled, non-inflammatory removal of damaged or unwanted

Cell Signalling · Growth FactorsGrowth Factors: Families, Receptors and Signalling PathwaysGrowth factors are secreted proteins that instruct cells whether to divide, differentiate, migrate or survive

Seroconversion Implications in SARS-CoV-2 Diagnostic Testing Key Points: COVID-19, the pandemic

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly

Explore 29 essential tips for crafting a compelling and well-structured PhD thesis, blending effective writing practices with time management and academic rigor for a successful dissertation journ

Western blotting is a powerful and widely used technique in molecular biology, essential for detecting and quantifying specific proteins in complex biological samples. However, despite its popu

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

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Sepsis, a life-threatening condition, is characterized by a dysregulated immune

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Imagine a future where a simple blood test could detect cancer in its earliest stages, long before symptoms appear. This isn't science fiction; it's the rapidly advancing reality of liquid biopsy, a r

The Assay Genie 3D Organoid Cell Viability Assay (AKES081) provided superior results compared to ThermoScientific PrestoBlue, maintaining over 100% viability at all time points in untrea

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Revolutionizing Research with AI Abstract Generator Explore how GPT can help in generating scientific abstracts based on figure legends and data. Discover the potential of AI in scientific

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MORV0013 functioned effectively in both qPCR machines and water baths, with or without paraffin oil, maintaining consistent sensitivity and specificity. MORV0013 performed reliably betwe

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The Dynex DS2 platform fully automates the MR-proANP and MR-proADM ELISA assays, streamlining sample processing from loading to detection. Automation ensures consistent assay performance

Workplace Testing for COVID-19 Corporate Testing for COVID-19 The impact of COVID-19 on the econom

Cell Cycle · KinasesCyclins and Cyclin-Dependent Kinases: Regulation, Substrates and Mitotic ControlCDKs are inactive on their own. They require a cyclin partner, and because cyclin levels rise and fa

Hepatic Function Assays Liver function assays are a suite of tests designed to assess the health and efficacy of our vital

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Stress and anxiety are a normal part of our daily lives and impact everything we do from our relationships with loved ones to our performance in the laboratory. Like any scientist, I experienced s

Cell Culture · ProtocolsCell Synchronisation Methods: Protocols for G1, S, G2 and M Phase ArrestAn asynchronous culture contains cells at every stage of the cycle at once, so any measurement is an ave

Oxidative Stress Definition Oxidative stress is a condition that results when the body produces or consumes more reactive oxygen species (ROS) than it can neutraliz

Techniques · Molecular CloningGuide to Bacterial Transformation: The Science of Genetic ManipulationBacterial transformation is the process by which bacteria take up foreign DNA — usually a plasmid —

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Acute pancreatitis (AP) is a condition in which activated enzymes and inflammatory mediators released from the pancreas cause damage to the pancreas and surrounding tissues. AP is furt

Haematology · CoagulationBlood Coagulation Pathway: Factors, Cascade and DisordersThe coagulation cascade is usually taught as two pathways converging on a common one. That framework maps neatly onto

Quick Facts About BococizumabWhat is Bococizumab?Bococizumab is a monoclonal antibody that targets PCSK9, a protein involved in cholesterol regulation.How Does Bococizumab Work?It inhibi

As a PhD student delving deep into the realm of cellular biology, there is one antagonist that consistently challenges my research: mycoplasma. This microscopic bacterium, with its a

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Immunophenotyping, a cornerstone of modern immunology, involves identifying and characterizing cell populations based on their surface markers. This technique provides insights into the heterog

Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—especi

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

If you've ever had a urinary tract infection, you know all about bacteria. These tiny organisms can invade your body and cause all sorts of problems. But what you may not know is tha

Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co

Introduction Cellular senescence is a fascinating phenomenon that plays a crucial role in the aging process and various age-related diseases. It is essential to explore the cellular

Schwann cells are cells that play a vital role in nerve function. They are responsible for myelinating axons and helping to ensure the transmission of electrical signals between neurons. Schwann c

Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem tha

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Mesenchymal stem cells (MSCs) have emerged as pivotal players in the realm of regenerative medicine due to their unique properties and versatile potential. Originally discovered in the bone marrow