Thioredoxin Reductase Activity Colorimetric Assay Kit (BN00976)

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SKU:
BN00976
  • Thioredoxin Reductase Activity Colorimetric Assay Kit (BN00976)
  • Thioredoxin Reductase Activity Colorimetric Assay Kit (BN00976)
€565

Description

ELISA Kit Technical ManualMSDS

Thioredoxin Reductase Activity Colorimetric Assay Kit

Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Assay Genie's Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (ax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

Figure: (a) Standard Curve (b) TrxR Kinetic Data

Key Information Description

Product SKU

BN00976

Size

100 assays

Detection Method

Absorbance (412 nm)

Species Reactivity

Mammalian

Applications

N/A

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • TrxR Assay Buffer
  • TNB Standard
  • DTNB
  • NADPH
  • TrxR Positive Control
  • TrxR Inhibitor

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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