Thioredoxin Reductase Activity Assay (BN00976)

SKU:
BN00976
  • Cell Biology Assays Thioredoxin Reductase Activity Assay BN00976
  • Cell Biology Assays Thioredoxin Reductase Activity Assay BN00976
€565

Description

Technical Manual MSDS

Thioredoxin Reductase Activity Assay

Assay Genie's Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (ax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

Thioredoxin Reductase Activity Assay Kit Data

Assay Information Description
Product Name: Thioredoxin Reductase Activity Assay
Product SKU: BN00976
Size: 100 assays
Detection Method: Colorimetric (Absorbance 412 nm)
Reactivity: Mammalian
Storage: Store the kit at -20°C, protect from light
Shipping Conditions: Gel Pack
Usage: For research use only. Not for use in humans.

Thioredoxin Reductase Activity Assay Kit Components

Component Quantity
TrxR Assay Buffer 25 ml
TNB Standard (lyophilized) 1 vial
DTNB (lyophilized) 1 vial
NADPH (lyophilized) 1 vial
TrxR Positive Control 1 vial
TrxR Inhibitor (lyophilized) 1 vial

Product Images

Figure: (a) Standard Curve (b) TrxR Kinetic Data

Kinase Background

Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.

View AllClose

Additional Information

Product type:
Cellular Assay
Detection Method:
Colorimetric
View AllClose