Triglyceride Quantification Colorimetric/Fluorometric Kit (BN00846)

(No reviews yet) Write a Review
SKU:
BN00846
  • Triglyceride Quantification Colorimetric/Fluorometric Kit (BN00846)
  • Triglyceride Quantification Colorimetric/Fluorometric Kit (BN00846)
€708

Description

ELISA Kit Technical ManualMSDS

Triglyceride Quantification Colorimetric/Fluorometric Kit

Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. Theglycerol is then oxidized to generate a product which reacts with theprobe to generate color (spectrophotometry at = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

Figure: (a) Triglyceride Standard Curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ) and assayed according to the kit protocol. Calculated concentration of triglyceride: 0.79 ± 0.1 mM; (70 ± 8.8 mg/dl).

Key Information Description

Product SKU

BN00846

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

  • The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples.
  • The kit also detects monoglycerides and diglycerides.

Features and Benefits

  • Simple procedure; takes ~1 hour
  • Fast and convenient
  • Kit contains the necessary reagents for accurate measurement of triglyceride in various biological samples

Kit Components

  • Triglyceride Assay Buffer
  • TriglycerideProbe (in DMSO, anhydrous)
  • Lipase
  • Triglyceride Enzyme Mix (lyophilized)
  • Triglyceride Standard (1 mM)

Storage Conditions

-20°C

Shitting Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose