ACTN2/ACTN3 Antibody (PACO23739)
- SKU:
- PACO23739
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ACTN2/ACTN3 Antibody (PACO23739) |
| Antibody SKU: | PACO23739 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from N-terminal of human Actin alpha -2/3. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from HeLa cells, HepG2 cells and HUVEC cells, using Actin alpha -2/3 antibody. |
 | Immunofluorescence analysis of HeLa cells, using Actin alpha -2/3 antibody. |
 | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin alpha -2/3 antibody. |
| Background: | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| Synonyms: | Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; |
| UniProt Protein Function: | ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 1q42-q43 Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; extracellular region; dendritic spine; actin filament; pseudopodium; Z disc; cytosol; filopodium Molecular Function:actin filament binding; protein dimerization activity; integrin binding; identical protein binding; protein binding; LIM domain binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity Biological Process: focal adhesion formation; platelet activation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; muscle filament sliding; protein homotetramerization; regulation of apoptosis; synaptic transmission; regulation of membrane potential; platelet degranulation; microspike biogenesis; blood coagulation; cell adhesion Disease: Cardiomyopathy, Dilated, 1aa |
| NCBI Summary: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P35609 |
| NCBI GenInfo Identifier: | 543742 |
| NCBI Gene ID: | 88 |
| NCBI Accession: | P35609.1 |
| UniProt Secondary Accession: | P35609,Q86TF4, Q86TI8, B1ANE4, B2RCS5, |
| UniProt Related Accession: | P35609 |
| Molecular Weight: | 103,920 Da |
| NCBI Full Name: | Alpha-actinin-2 |
| NCBI Synonym Full Names: | actinin, alpha 2 |
| NCBI Official Symbol: | ACTN2Â Â |
| NCBI Official Synonym Symbols: | CMD1AAÂ Â |
| NCBI Protein Information: | alpha-actinin-2; F-actin cross-linking protein; alpha-actinin skeletal muscle |
| UniProt Protein Name: | Alpha-actinin-2 |
| UniProt Synonym Protein Names: | Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN2Â Â |
| UniProt Entry Name: | ACTN2_HUMAN |
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