Anti-ANAPC4 Antibody (PACO18570)

Antibody Name:	ANAPC4 Antibody (PACO18570)
Antibody SKU:	PACO18570
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Synthetic peptide of human ANAPC4

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO18570(ANAPC4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HepG2 cells, 231 cells, hela cells, Primary antibody: PACO18570(ANAPC4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO18570(ANAPC4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans.

Synonyms:

anaphase promoting complex subunit 4

UniProt Protein Function:	APC4: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC4 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol Molecular Function:protein binding; ubiquitin-protein ligase activity; protein phosphatase binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
NCBI Summary:	A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Code:	Q9UJX5
NCBI GenInfo Identifier:	205371737
NCBI Gene ID:	29945
NCBI Accession:	Q9UJX5.2
UniProt Related Accession:	Q9UJX5
Molecular Weight:
NCBI Full Name:	Anaphase-promoting complex subunit 4
NCBI Synonym Full Names:	anaphase promoting complex subunit 4
NCBI Official Symbol:	ANAPC4
NCBI Official Synonym Symbols:	APC4
NCBI Protein Information:	anaphase-promoting complex subunit 4
UniProt Protein Name:	Anaphase-promoting complex subunit 4
UniProt Synonym Protein Names:	Cyclosome subunit 4
Protein Family:	Acetophenone carboxylase
UniProt Gene Name:	ANAPC4
UniProt Entry Name:	APC4_HUMAN

Antibodies

ANAPC4 Antibody (PACO07728)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)