Description
COVID-19 Human IgM PharmaGenie ELISA Kit - Kit Data
| Product SKU: | SBRS1733 |
| Size: | 96T |
| Gene Names: | IGM |
| Target Species: | Human |
| Compatible Sample Types: | Serum |
| Design Principle: | Indirect |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative / Semi-Quantitative |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C. |
Kit Components:
| 1. | SARS-CoV-2 N-protein coated 96-well microplate |
| 2. | Wash Buffer |
| 3. | Positive Control |
| 4. | Negative Control |
| 5. | HRP-anti positive control IgM Antibody |
| 6. | HRP-anti Human IgM Antibody |
| 7. | TMB One-Step Substrate |
| 8. | Stop Solution |
| 9. | Assay Diluent |
Other materials and equipment required:
The Assay Genie COVID-19 Human IgM ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed. |
| 2. | Add 100 µl positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature. |
| 3. | Add 100 µl prepared HRP-anti positive control IgM Antibody and/or 100 µl of prepared HRP-anti Human IgM Antibody to each well. Incubate 1 hour at room temperature. |
| 4. | Add 100 ul TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature. |
| 5. | Add 50 ul Stop Solution to each well. Read at 450 nm immediately. |
