Anti-Crotonyl-HIST1H2BC (K20) Antibody (PACO60477)

Antibody Name:	Crotonyl-HIST1H2BC (K20) Antibody (PACO60477)
Antibody SKU:	PACO60477
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IF, ChIP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:100-1:1000, IF:1:10-1:100
Species Reactivity:	Human
Immunogen:	Peptide sequence around site of Crotonyl-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Detected samples: K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM Sodium crotonylate for 4h. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
	Chromatin Immunoprecipitation Hela (10⁶, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (PACO60477) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.
	Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with PACO60477 at 1:12.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms:

Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK

UniProt Protein Function:	H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:	P62807
NCBI GenInfo Identifier:	290457686
NCBI Gene ID:	8339
NCBI Accession:	P62807.4
UniProt Secondary Accession:	P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:	P62807
Molecular Weight:	13,906 Da
NCBI Full Name:	Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:	histone cluster 1, H2bg
NCBI Official Symbol:	HIST1H2BG
NCBI Official Synonym Symbols:	H2B/a; H2BFA; H2B.1A; dJ221C16.8
NCBI Protein Information:	histone H2B type 1-C/E/F/G/I
UniProt Protein Name:	Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:	Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:	Histone
UniProt Gene Name:	HIST1H2BC
UniProt Entry Name:	H2B1C_HUMAN

Antibodies

Anti-Ubiquityl-Histone H2B-K120 Antibody (CAB17949)

Anti-HIST1H2BG Antibody (CAB9896)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)