Anti-Histone H2B Antibody (PACO23763)

Antibody Name:	Histone H2B Antibody (PACO23763)
Antibody SKU:	PACO23763
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500
Species Reactivity:	Human, Mouse
Immunogen:	Synthesized peptide derived from internal of human Histone H2B.

Form:	Liquid
Storage Buffer:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot analysis of extracts from A549 cells, using Histone H2B antibody.
	Immunofluorescence analysis of HeLa cells, using Histone H2B antibody.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using Histone H2B antibody.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms:

Histone H2B type 1-H; H2B.j; H2B/j; HIST1H2BH; H2BFJ

UniProt Protein Function:	H2B1H: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: DNA bindingBiological Process: nucleosome assembly
UniProt Protein Details:
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
UniProt Code:	Q93079
NCBI GenInfo Identifier:	7387739
NCBI Gene ID:	8345
NCBI Accession:	Q93079.3
UniProt Secondary Accession:	Q93079,Q4VB74, B2R541
UniProt Related Accession:	Q93079
Molecular Weight:	13,892 Da
NCBI Full Name:	Histone H2B type 1-H
NCBI Synonym Full Names:	histone cluster 1, H2bh
NCBI Official Symbol:	HIST1H2BH
NCBI Official Synonym Symbols:	H2B/j; H2BFJ
NCBI Protein Information:	histone H2B type 1-H
UniProt Protein Name:	Histone H2B type 1-H
UniProt Synonym Protein Names:	Histone H2B.j; H2B/j
Protein Family:	Histone
UniProt Gene Name:	HIST1H2BH
UniProt Entry Name:	H2B1H_HUMAN

Antibodies

Histone H2B Antibody (PACO00912)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)