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Human, Mouse and Rat Phospho-HDAC2 (S394) PharmaGenie ELISA Kit (SBRS1848)

SKU:
SBRS1848
Product Type:
ELISA Kit
Reactivity:
Human
Mouse
Rat
Applications:
ELISA
  • Human, Mouse and Rat Phospho-HDAC2 S394 PharmaGenie ELISA Kit SBRS1848
  • Human, Mouse and Rat Phospho-HDAC2 S394 PharmaGenie ELISA Kit SBRS1848
€760
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Description

Human, Mouse and Rat Phospho-HDAC2 (S394) PharmaGenie ELISA Kit (SBRS1848)

Product SKU:SBRS1848
Size:96T
Application:This ELISA kit recognizes Human.
Uniprot:F7ENH8 / P70288 / Q92769
Gene ID:3066
Gene Names:HDAC2
Synonyms:Histone deacetylase 2 (HD2) (EC 3.5.1.98)
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human, Mouse and Rat Phospho-HDAC2 (S394) PharmaGenie ELISA Kit (SBRS1848) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageHeLa cells were treated or untreated with Calyculin A. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageHeLa cells were treated with Calyculin A. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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