LIMD2 Antibody (PACO60761)
- SKU:
- PACO60761
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | LIMD2 Antibody (PACO60761) |
Antibody SKU: | PACO60761 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human LIM domain-containing protein 2 protein (1-127AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO60761 diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of SH-SY5Y cells with PACO60761 at 1:167, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO60761 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Acts as an activator of the protein-kinase ILK, thereby regulating cell motility. |
Synonyms: | LIM domain-containing protein 2, LIMD2 |
UniProt Protein Function: | LIMD2: Protein type: Unknown functionChromosomal Location of Human Ortholog: 17q23.3Molecular Function: protein binding |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | Q9BT23 |
NCBI GenInfo Identifier: | 74752322 |
NCBI Gene ID: | 80774 |
NCBI Accession: | Q9BT23.1 |
UniProt Secondary Accession: | Q9BT23,Q96S91, D3DU16 |
UniProt Related Accession: | Q9BT23 |
Molecular Weight: | 14,070 Da |
NCBI Full Name: | LIM domain-containing protein 2 |
NCBI Synonym Full Names: | LIM domain containing 2 |
NCBI Official Symbol: | LIMD2Â Â |
NCBI Official Synonym Symbols: | |
NCBI Protein Information: | LIM domain-containing protein 2 |
UniProt Protein Name: | LIM domain-containing protein 2 |
UniProt Synonym Protein Names: | |
Protein Family: | LIM domain-containing protein |
UniProt Gene Name: | LIMD2Â Â |
UniProt Entry Name: | LIMD2_HUMAN |
Antibodies |
LIMD2 Antibody (PACO10255) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |