MMP28 Antibody (PACO56194)
- SKU:
- PACO56194
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | MMP28 Antibody (PACO56194) |
| Antibody SKU: | PACO56194 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Matrix metalloproteinase-28 protein (351-475AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: MMP28 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 15 kDa. Observed band size: 59 kDa. |
 | IHC image of PACO56194 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO56194 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Can degrade casein. Could play a role in tissues homeostasis and repair. |
| Synonyms: | Matrix metalloproteinase-28 (MMP-28) (EC 3.4.24) (Epilysin), MMP28, MMP25 |
| UniProt Protein Function: | MMP28: Can degrade casein. Could play a role in tissues homeostasis and repair. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Protease; EC 3.4.24.-; Secreted; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q12 Cellular Component: proteinaceous extracellular matrix; cytoplasm Molecular Function:zinc ion binding; metalloendopeptidase activity; calcium ion binding Biological Process: proteolysis |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. This gene encodes a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q9H239 |
| NCBI GenInfo Identifier: | 37538314 |
| NCBI Gene ID: | 79148 |
| NCBI Accession: | Q9H239.2 |
| UniProt Secondary Accession: | Q9H239,Q96F04, Q96TE2, |
| UniProt Related Accession: | Q9H239 |
| Molecular Weight: | 520 |
| NCBI Full Name: | Matrix metalloproteinase-28 |
| NCBI Synonym Full Names: | matrix metallopeptidase 28 |
| NCBI Official Symbol: | MMP28Â Â |
| NCBI Official Synonym Symbols: | MM28; MMP25; MMP-25; MMP-28; EPILYSINÂ Â |
| NCBI Protein Information: | matrix metalloproteinase-28; matrix metalloprotease MMP25 |
| UniProt Protein Name: | Matrix metalloproteinase-28 |
| UniProt Synonym Protein Names: | Epilysin |
| Protein Family: | Matrix metalloproteinase |
| UniProt Gene Name: | MMP28Â Â |
| UniProt Entry Name: | MMP28_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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