HDL and LDL/VLDL Assay Kit – Information
Assay Genie’s HDL and LDL/VLDL cholesterol assay kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
HDL and LDL/VLDL Assay Kit – Key Features
- Sensitive and accurate. Requires only 20 μL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.
- Convenient. Room temperature assay. No 37°C heater is needed.
HDL and LDL/VLDL Assay Kit – Data Sheet
|Kit Includes||PBS: 1.5 mL Precipitation Reagent: 1.5 mL Assay Buffer: 20 mL Enzyme Mix: 120 uL NAD Solution: 2 mL Standard: 1 mL 300mg/dL cholesterol|
|Kit Requires||Pipetting (multi-channel) devices, clear bottom 96-well plate and plate reader|
|Method of Detection||OD340nm|
|Detection Limit||5 mg/dL|
|Protocol Length||60 min|
|Storage||Store PBS and Precipitation Reagent at room temperature and the rest reagents at -20°C.|
|Shelf Life||6 months|
Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable.