Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
The Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit is specifically designed for the quantitative detection of GFAP levels in rat serum, plasma, and tissue homogenates. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for your research needs.GFAP is a key protein found in astrocytes, the most abundant cells in the central nervous system. Changes in GFAP levels are associated with various neurological disorders, including traumatic brain injury, Alzheimer's disease, and multiple sclerosis. By measuring GFAP levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Whether you are studying neurodegenerative diseases or investigating the impact of brain injuries, the Rat GFAP ELISA Kit from Assay Genie is a valuable tool for your research. Get reliable and reproducible results to advance your understanding of GFAP biology and its implications in neurological disorders.
Product Name:
Rat GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
SKU:
RTES00998
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
