Recombinant Human AMBP/Alpha 1 Microglobulin Protein (His Tag) (RPES4706)



ELISA Kit Technical Manual

Recombinant Human AMBP/Alpha 1 Microglobulin Protein (His Tag)

Background:Protein AMBP belongs to the calycin superfamily and Lipocalin family. AMBP can be cleaved into three chains: ?-microglobulin, inter-?-trypsin inhibitor light chain and trypstatin. AMBP is expressed by the liver and secreted in plasma. ?-microglobulin occurs in many physiological fluids including the plasma, urine, and cerebrospinal fluid. Inter-?-trypsin inhibitor is present in the plasma and urine. ?-microglobulin occurs as a monomer and also in complexes with IgA and albumin, Inter-?-trypsin inhibitor inhibits trypsin, plasmin and lysosomal granulocytic elastase. Trypstatin act as a trypsin inhibitor, exists in a monomer forms and also occurs as a complex with tryptase in mast cells.
Name:Recombinant Human AMBP/Alpha 1 Microglobulin Protein (His Tag)
Assay Genie SKU:RPES4706
Synonyms:Protein AMBP; Alpha-Microglobulin; Protein HC; Alpha Microglycoprotein; Complex-Forming Glycoprotein Heterogeneous in Charge; Inter-Alpha-Trypsin Inhibitor Light Chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-Acid-Rich Protein; Trypstatin; AMBP; HCP; ITIL;A1M;EDC1;HCP;HI30;IATIL;ITI;ITIL;ITILC;UTI
Expression host:Human Cells
Mol Mass:21.9 kDa
AP Mol Mass:33 kDa
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; plasma membrane; extracellular region

Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; calcium oxalate binding; IgA binding; heme binding; calcium channel inhibitor activity

Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage

NCBI Summary:This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]
UniProt Code:P02760
NCBI GenInfo Identifier:122801
NCBI Gene ID:259
NCBI Accession:P02760.1
UniProt Related Accession:P02760
Molecular Weight:
NCBI Full Name:Protein AMBP
NCBI Synonym Full Names:alpha-microglobulin/bikunin precursor
NCBI Official Symbol:AMBP  
NCBI Official Synonym Symbols:A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC  
NCBI Protein Information:protein AMBP
UniProt Protein Name:Protein AMBP
UniProt Synonym Protein Names:Alpha microglycoprotein; Complex-forming glycoprotein heterogeneous in charge
Protein Family:Alpha-macroglobulin
UniProt Gene Name:AMBP  
UniProt Entry Name:AMBP_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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