Recombinant Human CAMK1G/CaMKI gamma Protein (His & GST Tag) (RPES0851)

SKU:
RPES0851
€626

Description

ELISA Kit Technical Manual

Recombinant Human CAMK1G/CaMKI gamma Protein (His & GST Tag)

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Additional Information

Background:Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKI? is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKI? membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKI? critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKI? specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKI? may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons. 
Name:Recombinant Human CAMK1G/CaMKI gamma Protein (His & GST Tag)
Size:20µg
Assay Genie SKU:RPES0851
Synonyms:CLICK3;CLICKIII;dJ272L16.1;RP1-272L16.2;VWS1
Species:Human
Expression host:Baculovirus-Insect Cells
Sequence:Met 1-Met 476
Accession:Q96NX5
Mol Mass:81 kDa
AP Mol Mass:75 kDa
Tag:N-His & GST
Purity:> 85 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT
Reconstitution:Please refer to the printed manual for detailed information.
Product type:
Recombinant Protein
Host species:
Baculovirus-Insect Cells
Reactivity:
Human
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