Recombinant Human Carboxypeptidase B2/CPB2 Protein (His Tag) (RPES4464)

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SKU:
RPES4464
€303

Description

ELISA Kit Technical Manual

Recombinant Human Carboxypeptidase B2/CPB2 Protein (His Tag)

Background:Carboxypeptidase B2 (CPB2) is a secreted enzyme that belongs to the peptidase M14 family. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen by the liver and circulates in the plasma as a plasminogen-bound zymogen. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides, such as kinins or anaphylatoxins, in the circulation regulating their activities. CPB2 also down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. CPB2 exhibits carboxypeptidase activity when it is activated by proteolysis at residue Arg92 of the thrombin/thrombomodulin complex. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Name:Recombinant Human Carboxypeptidase B2/CPB2 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4464
Synonyms:Carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma Carboxypeptidase B; pCPB; Thrombin-Activable Fibrinolysis Inhibitor; TAFI; CPB2
Species:Human
Expression host:Human Cells
Sequence:Phe23-Val423
Accession:Q96IY4
Mol Mass:47.0 kDa
AP Mol Mass:58 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl,150mM NaCl,1mM ZnCl2,10%Glycerol,pH7.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.17.20; Secreted, signal peptide; Secreted; Protease

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: extracellular space

Molecular Function:zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to drug; fibrinolysis; negative regulation of fibrinolysis; response to heat; blood coagulation; proteolysis

NCBI Summary:Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
UniProt Code:Q96IY4
NCBI GenInfo Identifier:317373332
NCBI Gene ID:1361
NCBI Accession:Q96IY4.2
UniProt Secondary Accession:Q96IY4,Q15114, Q5T9K1, Q5T9K2, Q9P2Y6, A8K464,
UniProt Related Accession:Q96IY4
Molecular Weight:423
NCBI Full Name:Carboxypeptidase B2
NCBI Synonym Full Names:carboxypeptidase B2 (plasma)
NCBI Official Symbol:CPB2  
NCBI Official Synonym Symbols:CPU; PCPB; TAFI  
NCBI Protein Information:carboxypeptidase B2; carboxypeptidase R; carboxypeptidase B-like protein; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase B2 (plasma, carboxypeptidase U)
UniProt Protein Name:Carboxypeptidase B2
UniProt Synonym Protein Names:Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI
Protein Family:Carboxypeptidase
UniProt Gene Name:CPB2  
UniProt Entry Name:CBPB2_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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