Human CD24 Recombinant Protein (RPES4934)

SKU:
RPES4934
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€101

Description

ELISA Kit Technical Manual

Recombinant Human CD24 Protein (Ser27-Gly59,Ser44Thr)(Fc Tag)

Background:Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.
Name:Recombinant Human CD24 Protein (Ser27-Gly59,Ser44Thr)(Fc Tag)
Size:10µg
Assay Genie SKU:RPES4934
Synonyms:Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; CD24; CD24A;FLJ22950;FLJ43543;MGC75043
Species:Human
Expression host:Human Cells
Sequence:Ser27-Gly59(Ser44Thr)
Accession:P25063
Mol Mass:29.8 kDa
AP Mol Mass:40-55 kDa
Tag:C-Fc
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.
UniProt Protein Details:

Protein type:Membrane protein, GPI anchor; Cell surface; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell surface; membrane; plasma membrane; lipid raft

Molecular Function:protein binding; signal transducer activity; protein tyrosine kinase activator activity; protein kinase binding

Biological Process: respiratory burst; axon guidance; cell migration; Wnt receptor signaling pathway; regulation of phosphorylation; B cell receptor transport into lipid raft; immune response-regulating cell surface receptor signaling pathway; chemokine receptor transport out of lipid raft; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; cell activation; cholesterol homeostasis; cell-cell adhesion; elevation of cytosolic calcium ion concentration; response to estrogen stimulus; response to molecule of bacterial origin; regulation of MAPKKK cascade; negative regulation of transforming growth factor-beta3 production; T cell costimulation; regulation of epithelial cell differentiation; response to hypoxia; regulation of cytokine and chemokine mediated signaling pathway

Disease: Multiple Sclerosis, Susceptibility To

UniProt Code:P25063
NCBI GenInfo Identifier:143811372
NCBI Gene ID:
NCBI Accession:P25063.2
Molecular Weight:
NCBI Full Name:Signal transducer CD24
UniProt Protein Name:Signal transducer CD24
UniProt Synonym Protein Names:Small cell lung carcinoma cluster 4 antigen; CD_antigen: CD24
Protein Family:Signal transducer
UniProt Gene Name:CD24  
UniProt Entry Name:CD24_HUMAN
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