Ephrin Type-B Receptor 1 (EPHB1) is a single-pass type I membrane protein that belongs to the Ephrin-B family of receptor tyrosine kinases that is involved in embryonic nervous and vascular system development. EPHB1/EPHT2 contains two fibronectin type-III domains; one protein kinase domain and one SAM (sterile ? motif) domain. EPHB1 could stimulate fibroblast motility on extracellular matrix in a kinase-dependent manner; which also correlated with its association with Grb7; an adaptor molecule implicated in the regulation of cell migration. It binds to ephrin-B1; ephrin-B2 and ephrin-B3. EPHB1 plays an important roles in diverse biological processes including nervous system development; angiogenesis; and neural synapsis formation and maturation and may be involved in cell-cell interactions in the nervous system.
Immobilized Human EphB1-His at 10µg/ml(100 ?l/well) can bind Mouse EFNB2-Fc(Cat: PKSM041012). The ED50 of Human EphB1-His is 53.1 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human EphB1 is produced by our Mammalian expression system and the target gene encoding Ser564-Ala984 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
48.8 kDa
AP Mol Mass:
49 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris;150mM NaCl;pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
