Recombinant Human LDH-B/LDHB Protein (His Tag) (RPES3674)

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SKU:
RPES3674
€361

Description

ELISA Kit Technical Manual

Recombinant Human LDH-B/LDHB Protein (His Tag)

Background:L-Lactate Dehydrogenase B Chain (LDH-B) is a member of the lactate dehydrogenase family that consists of three members, LDH-A, LDH-B and LDH-C; members of this family function as powerful markers for germ cell tumors. LDH-B is an oxidoreductase that catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate to lactate when oxygen is absent or in short supply and it performs the reverse reaction during the Cori cycle in the liver. It is also called Hydroxybutyrate Dehydrogenase (HBD) due to its ability to catalyze the oxidation of hydroxybutyrate.
Name:Recombinant Human LDH-B/LDHB Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES3674
Synonyms:L-lactate Dehydrogenase B Chain; LDH-B; LDH Heart Subunit; LDH-H; Renal Carcinoma Antigen NY-REN-46; LDHB
Species:Human
Expression host:E.coli
Sequence:Met 1-Leu334
Accession:P07195
Mol Mass:38.8 kDa
AP Mol Mass:37 kDa
Tag:N-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl, 100mM NaCl, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
NCBI Summary:This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
UniProt Code:P07195
NCBI GenInfo Identifier:126041
NCBI Gene ID:3945
NCBI Accession:P07195.2
UniProt Related Accession:P07195
Molecular Weight:~ 37kDa
NCBI Full Name:L-lactate dehydrogenase B chain
NCBI Synonym Full Names:lactate dehydrogenase B
NCBI Official Symbol:LDHB  
NCBI Official Synonym Symbols:LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281  
NCBI Protein Information:L-lactate dehydrogenase B chain
UniProt Protein Name:L-lactate dehydrogenase B chain
UniProt Synonym Protein Names:LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:LDHB  
UniProt Entry Name:LDHB_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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