AAK1 Antibody (PACO48618)
- SKU:
- PACO48618
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | AAK1 Antibody (PACO48618) |
| Antibody SKU: | PACO48618 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human AP2-associated protein kinase 1 protein (274-465AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Mouse kidney tissue. All lanes: AAK1 antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 104, 94 kDa. Observed band size: 104 kDa.. |
 | Immunofluorescent analysis of HepG2 cells using PACO48618 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human brain tissue using PACO48618 at dilution of 1:100. |
| Background: | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. |
| Synonyms: | AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1), AAK1, KIAA1048 |
| UniProt Protein Function: | AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; NAK family Chromosomal Location of Human Ortholog: 2p14 Cellular Component: clathrin-coated vesicle; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button Molecular Function:Notch binding; protein binding; protein serine/threonine kinase activity Biological Process: positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization |
| NCBI Summary: | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q2M2I8 |
| NCBI GenInfo Identifier: | 300669613 |
| NCBI Gene ID: | 22848 |
| NCBI Accession: | Q2M2I8.3 |
| UniProt Secondary Accession: | Q2M2I8,Q4ZFZ3, Q53RX6, Q9UPV4, |
| UniProt Related Accession: | Q2M2I8 |
| Molecular Weight: | 93,578 Da |
| NCBI Full Name: | AP2-associated protein kinase 1 |
| NCBI Synonym Full Names: | AP2 associated kinase 1 |
| NCBI Official Symbol: | AAK1Â Â |
| NCBI Protein Information: | AP2-associated protein kinase 1 |
| UniProt Protein Name: | AP2-associated protein kinase 1 |
| UniProt Synonym Protein Names: | Adaptor-associated kinase 1 |
| Protein Family: | AP2-associated protein kinase |
| UniProt Gene Name: | AAK1Â Â |
| UniProt Entry Name: | AAK1_HUMAN |
| Antibodies | ELISA Kits |
| AAK1 Antibody (PACO00349) | Human AAK1 (AP2-associated protein kinase 1) ELISA Kit (HUFI05483) |
| AAK1 Colorimetric Cell-Based ELISA |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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