Anti-ACTG1 Antibody (PACO47746)

Antibody Name:	ACTG1 Antibody (PACO47746)
Antibody SKU:	PACO47746
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Actin, cytoplasmic 2 protein (13-166AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate. All lanes: ACTG1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 kDa. Observed band size: 42 kDa..
	Immunofluorescent analysis of Hela cells using PACO47746 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human breast cancer using PACO47746 at dilution of 1:100.

Background:	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Synonyms:	Actin, cytoplasmic 2 (Gamma-actin) [Cleaved into: Actin, cytoplasmic 2, N-terminally processed], ACTG1, ACTG

UniProt Protein Function:	ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 17q25 Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; nucleus; cytosol Molecular Function:identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding Biological Process: axon guidance; retinal homeostasis; intercellular junction assembly and maintenance; ephrin receptor signaling pathway; innate immune response; sarcomere organization; cell motility; vascular endothelial growth factor receptor signaling pathway Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20
NCBI Summary:	Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]
UniProt Code:	P63261
NCBI GenInfo Identifier:	54036678
NCBI Gene ID:	71
NCBI Accession:	P63261.1
UniProt Secondary Accession:	P63261,P02571, P14104, P99022, Q5U032, Q96E67, A8K7C2
UniProt Related Accession:	P63261
Molecular Weight:	375
NCBI Full Name:	Actin, cytoplasmic 2
NCBI Synonym Full Names:	actin, gamma 1
NCBI Official Symbol:	ACTG1
NCBI Official Synonym Symbols:	ACT; ACTG; BRWS2; DFNA20; DFNA26; HEL-176
NCBI Protein Information:	actin, cytoplasmic 2; cytoskeletal gamma-actin; epididymis luminal protein 176
UniProt Protein Name:	Actin, cytoplasmic 2
UniProt Synonym Protein Names:	Gamma-actinActin, cytoplasmic 2, N-terminally processed
Protein Family:	Actin
UniProt Gene Name:	ACTG1
UniProt Entry Name:	ACTG_HUMAN

Antibodies	ELISA Kits
ACTG1 Antibody (PACO07580)	Human ACTG1 / Gamme Actin ELISA Kit
	Human ACTG1 (Actin Gamma 1) CLIA Kit (HUES00190)
	Human ACTG1 (Actin Gamma 1) ELISA Kit (HUES01494)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)