Antibody Arrays
Screen 7 to 274 proteins from a single sample on one membrane, using the western blotting and chemiluminescent imaging equipment already in your lab. Built for biomarker discovery, pathway profiling and target validation.
Cytokine arrays from 23 to 274 targets, plus inflammation, chemokine, growth factor, angiogenesis, apoptosis, immune checkpoint, neuro and cancer discovery arrays.
Mouse cytokine arrays from 22 to 144 targets, with chemokine, growth factor, inflammation, apoptosis, angiogenesis, neuro and immune checkpoint arrays for both species.
Bovine, canine, porcine, sheep, equine, chicken and dolphin cytokine, chemokine, interleukin, inflammation and T cell response arrays.
Each array is a membrane pre-spotted with capture antibodies, one position per target. The sample is incubated on the membrane and each protein is trapped by its matched capture antibody. Bound proteins are detected with a cocktail of biotinylated antibodies, followed by streptavidin-HRP and a chemiluminescent substrate. Spot intensity is proportional to the amount of protein and is quantified by densitometry.
The read-out is semi-quantitative: it shows which proteins change between samples and by how much, relative to a control. That makes an array a fast way to reach a shortlist of candidates from a whole pathway or disease signature, which can then be confirmed and measured in absolute terms by ELISA.
Cytokine, chemokine, interleukin, inflammation, Th1/Th2/Th17 and T cell response arrays for immune profiling.
Apoptosis, autophagy, DNA damage response, NF-kB, TGF beta, BMP, heat shock protein and stem cell arrays.
Cancer discovery, immune checkpoint, angiogenesis, atherosclerosis, obesity, acute kidney injury, neuro discovery, MMP and periodontal disease arrays.
Add serum, plasma, lysate or culture supernatant to the blocked membrane.
Apply the biotinylated detection antibody cocktail.
Add streptavidin-HRP and the chemiluminescent substrate.
Capture on a chemiluminescent imager and quantify spot density.
A membrane carrying a grid of capture antibodies, each specific for a different protein. One incubation with the sample reports on every target on the membrane at once.
Between 7 and 274, depending on the array. The number of targets is given in each product name.
It is semi-quantitative. Arrays show relative changes in protein levels between samples. Use an ELISA to measure absolute concentrations of the hits.
Serum, plasma, cell culture supernatants, cell lysates and tissue lysates. Check the product page for the samples validated for each array.
A standard western blotting set-up and a chemiluminescent imaging system. No dedicated array reader is required.
Kits contain 2, 4 or 8 membranes. Each sample needs its own membrane, so count your samples and controls and include a replicate where you can.
Confirm candidates with a quantitative ELISA for each protein, or follow a set of them with a multiplex panel.