E.Coli Dnak Lid Covering Substrate Recombinant Protein (CPRT5214)

SKU:
CPRT5214
€278

Description

Data SheetMSDS

DnaK Lid Covering Substrate E.Coli Recombinant Protein

Name:DnaK Lid Covering Substrate E.Coli
Synonyms:HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.
Introduction:DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the foldingand assembly of newly synthesized polypeptide chains and in preventingthe aggregation of stress–denatured proteins. Dnak(residues 508–638) of the substrate bindingdomain is a–helical and appears to act as a lid coveringthe substrate bindingcleft. DnaK(amino acid 508–638) was purified to apparent homogeneity by usingconventional column chromatography techniques. Additional amino acid (Met) is attached at N– terminus.
Description:Recombinant DnaK Lid CoveringSubstrate domain produced in E.Coli is a single, non–glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 14.6 kDa.
Source:Escherichia Coli.
Pack size:100µg
Physical Appearance:Sterile filtered colorless solution.
Formulation:The DnaK protein contains 25mM Tris–HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze–thaw cycles.
Purity:Greater than 95.0% as determined by(a) Analysis by RP–HPLC.(b) Analysis by SDS–PAGE.
Amino Acid Sequence:MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIESALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADASANNAKDDDVVDAEFEEVKDKK.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
View AllClose

Additional Information

Product type:
Recombinant Protein
Reactivity:
E.Coli
Host Species:
E.Coli
View AllClose