Human NUDT1 Recombinant Protein (RPPB2043)
- SKU:
- RPPB2043
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P36639
- Research Area:
- Enzymes
Description
| Product Name: | Human NUDT1 Recombinant Protein |
| Product Code: | RPPB2043 |
| Size: | 20µg |
| Species: | Human |
| Target: | NUDT1 |
| Synonyms: | 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, Nucleoside diphosphate-linked moiety X motif 1, Nudix motif 1, NUDT1, MTH1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NUDT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV |
NUDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. NUDT1 is found mostly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Moreover, NUDT1 is expressed at much higher levels in proliferating cells than in resting cells. Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations which may result in carcinogenesis or neurodegeneration.
NUDT1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 176 amino acids (1-156 a.a.) and having a molecular mass of 20.1kDa. The NUDT1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:EC 3.6.1.55; Hydrolase; EC 3.6.1.56 Chromosomal Location of Human Ortholog: 7p22 Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol Molecular Function:GTPase activity; snoRNA binding; m7G(5')pppN diphosphatase activity; metal ion binding; ATP diphosphatase activity; 8-oxo-7,8-dihydroguanine triphosphatase activity Biological Process: DNA protection; dGTP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; dATP catabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair |
| NCBI Summary: | Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36639 |
| NCBI GenInfo Identifier: | 254763430 |
| NCBI Gene ID: | 4521 |
| NCBI Accession: | P36639.3 |
| UniProt Secondary Accession: | P36639,Q6LES7, Q6P0Y6, Q7Z7N6, Q8IV95, Q9UBM0, Q9UBM9 A4D205, |
| UniProt Related Accession: | P36639 |
| Molecular Weight: | 197 |
| NCBI Full Name: | 7,8-dihydro-8-oxoguanine triphosphatase |
| NCBI Synonym Full Names: | nudix (nucleoside diphosphate linked moiety X)-type motif 1 |
| NCBI Official Symbol: | NUDT1�� |
| NCBI Official Synonym Symbols: | MTH1�� |
| NCBI Protein Information: | 7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; nudix motif 1; mutT human homolog 1; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1 |
| UniProt Protein Name: | 7,8-dihydro-8-oxoguanine triphosphatase |
| UniProt Synonym Protein Names: | 2-hydroxy-dATP diphosphatase (EC:3.6.1.56); 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1 |
| Protein Family: | Nudix hydrolase |
| UniProt Gene Name: | NUDT1�� |
| UniProt Entry Name: | 8ODP_HUMAN |
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